LAY, A New Endoplasmic Reticulum Retention Signal for UBIAD1 Protein
نویسندگان
چکیده
In this paper, we observed the co-localization of UBIAD1 and Ds Red-ER (an ER marker) in bladder carcinoma cell line T24 and human embryonic kidney cell line HEK293T. UBIAD1 accumulated on the endoplasmic reticulum (ER) in both T24 and HEK293T cell lines. Site-directed mutagenesis defined a LAY motif, positioned on 71-73 amino acids of the UBIAD1 N-terminus, as the ER retention signal of UBIAD1. Based upon flow cytometry analysis, it is shown that mutations of LAY motif increased the UBIAD1-induced apoptosis of T24 cells. Disruption of the UBIAD1 localization on ER influenced its tumor suppressant activity.
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